Long non-coding RNA XIST promotes proliferation, autophagy and inhibits apoptosis by regulating microRNA-30c/ATG5 axis in gastric cancer

摘要: Background: Gastric cancer (GC) is a great threat to human health and life. Long non-coding RNA X inactive-specific transcript (XIST) microRNA-30c (miR-30c) function as crucial players in the tumorigenesis of GC. Bioinformatics analysis suggests that miR-30c has chance interact with XIST autophagy related 5 (ATG5). Moreover, ATG5 been identified target intestinal epithelial cells. Hence, whether could regulate cell proliferation, apoptosis by miR-30c/ATG5 axis was further investigated Methods: The levels XIST, mRNA were measured RT-qPCR assay. ATG5, p62, LC3-I, LC3-II protein expression detected western blot relationships examined luciferase, immunoprecipitation (RIP) pull down assays. Cell proliferation assessed MTS apoptotic rate determined using flow cytometry. tissues immunohistochemistry (IHC) Results: highly expressed GC lines. knockdown suppressed promoted inhibited direct interaction Furthermore, depletion abrogated deficiency-mediated anti-proliferation, pro-apoptosis anti-autophagy effects Additionally, sequestering from Conclusion: induced through regulating cells, hinting at potential value management