CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging
作者： Hanhui Ma , Li-Chun Tu , Ardalan Naseri , Yu-Chieh Chung , David Grunwald
DOI: 10.1038/S41592-018-0174-0
关键词: RNA 、 Computational biology 、 Biology 、 Genome 、 Live cell imaging 、 Gene 、 Guide RNA 、 Aptamer 、 CRISPR 、 Binding site
摘要: Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins enhance brightness live cell imaging of genomic loci. However, many these modifications result in instability and thus produce lower genome-labeling efficiency than anticipated. Here we introduce CRISPR-Sirius, based on octet arrays aptamers conferring both enhanced stability brightness, provide initial biological applications this platform.
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