Novel transporters from Kluyveromyces marxianus and Pichia guilliermondii expressed in Saccharomyces cerevisiae enable growth on l‐arabinose and d‐xylose

摘要: Genes encoding l-arabinose transporters in Kluyveromyces marxianus and Pichia guilliermondii were identified by functional complementation of Saccharomyces cerevisiae whose growth on was dependent a functioning transporter, or screening differential display library, respectively. These also transport d-xylose designated KmAXT1 (arabinose–xylose transporter) PgAXT1, Transport assays using showed that KmAxt1p has Km 263 mm Vmax 57 nm/mg/min, PgAxt1p 0.13 mm 18 nm/mg/min. Glucose, galactose xylose significantly inhibit both transporters. 27 mm 3.8 nm/mg/min, 65 mm 8.7 nm/mg/min. Neither transporter is capable recovering glucose S. strain deleted for hexose kinetics Gal2p 371 mm 341 nm/mg/min l-arabinose, 25 mm 76 nm/mg/min galactose. Due to the ability these two newly characterized d-xylose, one scenario complete usage biomass-derived pentose sugars would require only low-affinity, high-throughput additional high-affinity general rather than dedicated Additionally, alignment with other provides potential targets substrate recognition engineering. Accession Nos: KmAXT1: GZ791039; PgAXT1: GZ791040 Copyright © 2015 John Wiley & Sons, Ltd.