Non-destructive assay systems for detection of β-glucuronidase activity in higher plants
作者： Thomas Martin , Renate Schmidt , Thomas Altmann , Wolf B. Frommer
DOI: 10.1007/BF02669263
关键词: Glucuronidase activity 、 Biochemistry 、 Biology 、 Proteomics 、 Metabolomics 、 Non destructive 、 Plant science 、 Molecular biology
摘要: β-glucuronidase (GUS) can be qualitatively assayed in seedlings and fully grown plants without injury or irreversible damage by short term incubations X-gluc spraying 4-MUG.
springer.com
sci-hub.se 参考文章(15)
J. Stockhaus, J. Schell, L. Willmitzer, Correlation of the expression of the nuclear photosynthetic gene ST-LS1 with the presence of chloroplasts. The EMBO Journal. ,vol. 8, pp. 2445- 2451 ,(1989) , 10.1002/J.1460-2075.1989.TB08379.X
Michael Keil, Jose J. Sánchez-Serrano, Lothar Willmitzer, Both wound-inducible and tuber-specific expression are mediated by the promoter of a single member of the potato proteinase inhibitor II gene family. The EMBO Journal. ,vol. 8, pp. 1323- 1330 ,(1989) , 10.1002/J.1460-2075.1989.TB03512.X
Mario Rocha-Sosa, Uwe Sonnewald, Wolf Frommer, Marina Stratmann, Jeff Schell, Lothar Willmitzer, Both developmental and metabolic signals activate the promoter of a class I patatin gene The EMBO Journal. ,vol. 8, pp. 23- 29 ,(1989) , 10.1002/J.1460-2075.1989.TB03344.X
Thomas Martin, Rosa-Valentina Wöhner, Sabine Hummel, Lothar Willmitzer, Wolf B. Frommer, The GUS reporter system as a tool to study plant gene expression. GUS protocols: using the GUS gene as a reporter of gene expression.. pp. 23- 43 ,(1992) , 10.1016/B978-0-12-274010-7.50008-2
G. Vancanneyt, R. Schmidt, A. O'Connor-Sanchez, L. Willmitzer, M. Rocha-Sosa, Construction of an intron-containing marker gene : splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation Molecular Genetics and Genomics. ,vol. 220, pp. 245- 250 ,(1990) , 10.1007/BF00260489
Richard A. Jefferson, Assaying chimeric genes in plants: The GUS gene fusion system Plant Molecular Biology Reporter. ,vol. 5, pp. 387- 405 ,(1987) , 10.1007/BF02667740
Jean H. Gould, Roberta H. Smith, A non-destructive assay for GUS in the media of plant tissue cultures Plant Molecular Biology Reporter. ,vol. 7, pp. 209- 216 ,(1989) , 10.1007/BF02668687
Richard WALDEN, Jeff SCHELL, Techniques in plant molecular biology – progress and problems FEBS Journal. ,vol. 192, pp. 563- 576 ,(1990) , 10.1111/J.1432-1033.1990.TB19262.X
Renate Schmidt, Lothar Willmitzer, High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants Plant Cell Reports. ,vol. 7, pp. 583- 586 ,(1988) , 10.1007/BF00272763
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X