Human microvasculature fabrication using thermal inkjet printing technology.

摘要: The current tissue engineering paradigm is that successfully engineered thick tissues must include vasculature. As biological approaches alone, such as VEGF, have fallen short of their promises, one may look for an approach to build microvasculature. Layer-by-layer customized fabrication cell/scaffold constructs shown some potential in building complex 3D structures. With the advent cell printing, be able precise human microvasculature with suitable bio-ink. Human microvascular endothelial cells (HMVEC) and fibrin were studied bio-ink construction. Endothelial are only compose capillaries also form entire inner lining cardiovascular system. Fibrin has been already widely recognized scaffold vasculature other cells, including skeleton/smooth muscle chondrocytes. In our study, we precisely fabricated micron-sized channels using a drop-on-demand polymerization. This printing technique uses aqueous processes induce little, if any, damage cells. When HMVEC conjunction fibrin, found aligned themselves inside proliferated confluent linings. tubular structure was printed patterns. We conclude combined simultaneous can promote proliferation formation.