First Report of Zebra Chip and 'Candidatus Liberibacter solanacearum' on Potatoes in Nicaragua.

摘要: In September 2011, potato (Solanum tuberosum) tubers grown in Nicaragua outside of Esteli and Jinotega were observed with internal discoloration suggestive zebra chip (ZC); the plants showed foliar symptoms chlorosis, leaf scorching, wilting, vascular discoloration, swollen nodes, twisted stems, aerial (3). Disease incidence ranged from 50 to 95% eight fields ranging 5 12 ha regions Nicaragua. Leaf samples psyllids collected two fields, total DNA was purified leaves 17 symptomatic 10 asymptomatic plants. also extracted 20 individual psyllids. Primers specific for 16S rDNA (OA2 OI2c) surface antigen gene (OMB 1482f 2086r) Candidatus Liberibacter solanacearum (CLs) used confirm presence pathogen infected potatoes insects (2). All tested positive CLs using both primers, no ha...