Motility of the diplomonad fish parasite Spironucleus vortens through thixotropic solid media

摘要: Investigation of a series nutrient-supplemented thixotropic gels at successive dilutions that impede the trajectories highly vigorous motile flagellated protist, Spironucleus vortens, provides insights into both its swimming characteristics and means for immobilization. The progress movement this organism through solidified growth medium was monitored by in situ reductive production formazan chromophore from dissolved tetrazolium salt. physical properties were measured using an Anton Paar rheometer. test parameters measurements included: angular frequency, complex viscosity, shear modulus, rate rotational recovery. These rheological affected forward velocity gels, during after multiple resetting, information potentially useful determination dynamic flagellar propulsion rates organism. Application to separation single cells, individuals distinct sizes or differing species mixed cultures non-motile organisms less actively evident. applications can be used when isolating parasite intestinal contents host faecal pellets.