Differential effect of tumour necrosis factor on human thymocyte subpopulations.

摘要: We have studied the effect of tumour necrosis factor (TNF) on purified human thymocyte subpopulations. For this purpose thymocytes were by negative selection with three rounds several antibodies plus complement. TNF was able to co-stimulate in a dose-response manner proliferation single positive (SP) CD3+ CD4+ or CD8+ presence optimal doses interleukin-2 (IL-2), phytohaemagglutinin (PHA), anti-CD3 phorbol esters. However, CD1+ CD3low cortical did not proliferate significantly response any stimulus alone combination. The proliferative SP blocked an anti-IL-2R alpha antibody. In addition, enhanced expression IL-2R but beta cell surface CD1- over levels induced other primary stimuli, inducing as consequence, increase number high affinity IL-2R. Furthermore, mRNA thymocytes. On hand, mitogenic absence for CD3- CD4- CD8- prethymocytes, IL-2, and antibodies. subset IL-2 additive. directly both chains, alpha, messenger RNA (mRNA) prethymocytes. summary, our results suggest that may important role co-stimulatory signal some subpopulations