Libraries of peptides and proteins displayed on filamentous phage

摘要: Publisher Summary A fusion phage is a filamentous virion displaying on its surface foreign peptide fused to coat protein and harboring the gene for within genome. This chapter emphasizes an application which these expression vectors are particularly well suited: construction of epitope libraries. In such library—the kind so far constructed—the phages display random peptides encoded by degenerate synthetic oligonucleotides spliced into gene, library as whole representing up billions sequences. Affinity purified eluted without destroying their infectivity, sequences responsible binding easily ascertained infecting bacteria, propagating resulting clones, sequencing relevant part viral DNAs. The number that can be accommodated with this technology exceeds factor 100–1,000 screened conventional systems, in not displayed propagatable unit encodes it. It also discusses more complex libraries, ligands folded domains.