Evaluation of Anthelmintic Activity and Composition of Pumpkin (Cucurbita pepo L.) Seed Extracts—In Vitro and in Vivo Studies
作者： Maciej Grzybek , Wirginia Kukula-Koch , Aneta Strachecka , Aleksandra Jaworska , Andrew M Phiri
DOI: 10.3390/IJMS17091456
关键词: Pumpkin seed 、 Botany 、 Palmatine 、 Anthelmintic 、 Hatching 、 Nematode 、 Cucurbitaceae 、 Biology 、 Food science 、 In vivo 、 Cucurbita pepo
摘要: A significant number of studies report growing resistance in nematodes thriving both humans and livestock. This study was conducted to evaluate the vitro vivo anthelmintic efficiency Curcubita pepo (C. pepo) L. hot water extract (HWE), cold (CWE) or ethanol (ETE) on two model nematodes: Caenorhabditis elegans elegans) Heligmosoides bakeri (H. bakeri). Methods: Raman, IR LC-MS spectroscopy analyses were performed studied plant material deliver qualitative quantitative data composition obtained extracts: ETE, HWE CWE. The activity evaluation showed an impact C. extracts different developmental stages H. bakeri. following experiments mice infected with confirmed inhibitory properties most active pumpkin selected by study. All found contain cucurbitine, aminoacids, fatty acids, and-for first time-berberine palmatine identified. seed exhibited a nematidicidal potential vitro, affecting survival L1 L2 larvae. ETE strongest demonstrated positive effect eggs hatching marked against worm motility, compared PBS control. No effects integrity motility found. EtOH fecal egg counts adult burdens. highest reduction observed for 8 g/kg dose (IC50 = 2.43; 95% Cl 2.01–2.94). decrease faecal (FEC) accompanied burden treated control group. Conclusions: Pumpkin may be used Gastrointestinal (G.I.) nematode infections. relatively inexpensive alternative currently available chemotherapeutic should considered as novel drug candidate nearest future.
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