Anti-androgenic and insulin-sensitizing actions of Nigella sativa oil improve polycystic ovary and associated dyslipidemia and redox disturbances

摘要: Aim: Nigella sativa oil has been shown to improve metabolic syndrome-associated dyslipidemia and oxidative stress. Effects of N. on letrozole-induced polycystic ovary syndrome (PCOS)-associated hormonal, metabolic, redox, lipid dysregulation in rats were investigated. Methods: Female Sprague-Dawley randomly assigned into four groups (n=10 per group): control, PCOS, PCOS treated with (5 mL/kg/day or 10 mL/kg/day) once daily p.o. starting at day 7 after the commencement letrozole (1mg/kg) until 56. Body weight estrous cycle recorded from days 28 respectively Rats dissected serum ovaries collected for biochemical histomorphological assessments. Results: (10 treatment significantly increased: number undergoing regular (80% vs 20%; p< 0.05), average cycles (5.00±0.59 0.20±0.42; appearance corpus luteum; but reduced cystic follicles (7.60±1.51 6.10±0.19; 0.05) rats. was also by However, ovarian weight, granulose theca cells layers showed no significant change. The restored altered circulating levels gonadotropins, steroids fasting blood glucose. total cholesterol, triglycerides, low density lipoprotein-cholesterol, malondialdehyde markedly while insulin sensitivity high superoxide dismutase glutathione peroxidase activities greatly increased. Conclusion: improved ovarian-morphology disorders via its anti-androgenic insulin-sensitizing actions ameliorate PCOS-associated redox disturbances.