Comparison of stratum corneum and hair fibrous proteins.

作者: Howard P. Baden , Norah McGilvray , Loretta D. Lee , Lynn Baden , Joseph Kubilus

DOI: 10.1111/1523-1747.EP12530930

关键词: UreaAmino acidMacromoleculeEpidermisCyanogen bromideBiochemistryPolyacrylamide gel electrophoresisStratum corneumElectrophoresisChemistry

摘要: The Tris-urea-mercaptoethanol (pH 9) soluble fibrous proteins of hair and stratum corneum have been compared in a number animals. 2 tissues show different urea 8.3) SDS polyacrylamide gel electrophoretic patterns vary amino acid composition. Cyanogen bromide cleavage was done on human found to give fragements by electrophoresis. Antibodies protein did not react with vice versa. Although the types same helical structure as shown x-ray diffraction analysis, their differences composition may be important for specific interactions other macromolecules.

参考文章(18)
H. Lindley, The Chemical Composition and Structure of Wool Springer, Boston, MA. pp. 147- 191 ,(1977) , 10.1007/978-1-4613-4109-3_4
R. F. Doolittle, K. W. K. Watt, B. A. Cottrell, D. D. Strong, M. Riley, The amino acid sequence of the α -chain of human fibrinogen Nature. ,vol. 280, pp. 464- 468 ,(1979) , 10.1038/280464A0
L D Lee, J Kubilus, H P Baden, Intraspecies heterogeneity of epidermal keratins isolated from bovine hoof and snout Biochemical Journal. ,vol. 177, pp. 187- 196 ,(1979) , 10.1042/BJ1770187
Joseph Kubilus, Martha J. Macdonald, Howard P. Baden, Epidermal proteins of cultured human and bovine keratinocytes Biochimica et Biophysica Acta. ,vol. 578, pp. 484- 492 ,(1979) , 10.1016/0005-2795(79)90178-8
L.D. Lee, B.C. Fleming, R.F. Waitkus, H.P. Baden, Isolation of the polypeptide chains of prekeratin. Biochimica et Biophysica Acta. ,vol. 412, pp. 82- 90 ,(1975) , 10.1016/0005-2795(75)90341-4
L D Lee, H P Baden, J. Kubilus, B F Fleming, Immunology Of Epidermal Fibrous Proteins Journal of Investigative Dermatology. ,vol. 67, pp. 521- 525 ,(1976) , 10.1111/1523-1747.EP12664546
Loretta D. Lee, Howard P. Baden, The use of denaturing conditions for gel diffusion of insoluble epidermal proteins Journal of Immunological Methods. ,vol. 18, pp. 381- 385 ,(1977) , 10.1016/0022-1759(77)90192-2
T.-T. Sun, C. Shih, H. Green, Keratin cytoskeletons in epithelial cells of internal organs. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 2813- 2817 ,(1979) , 10.1073/PNAS.76.6.2813