Tumor necrosis factor-alpha production of influenza A virus-infected macrophages and potentiating effect of lipopolysaccharides.

摘要: Influenza A virus infections are commonly associated with symptoms that suggest involvement of TNF-alpha. In this study, we exposed human monocytes, rat alveolar macrophages, and murine PU5-1.8 macrophages to influenza virus, strain Puerto Rico 8. We observed a productive infection was accompanied by TNF-alpha mRNA accumulation, release subsequent cell death. production dependent on exposure live in contrast IFN also induced UV-inactivated virus. Most strikingly, low amounts LPS (1 10 ng/ml) from Escherichia coli or Haemophilus influenzae were capable strongly potentiating virus-infected macrophages. The effect neither due increased survival nor altered multiplication, enhanced gene expression, discharge intracellular stores, shifts the kinetics release. Thus, LPS, which could easily be present vivo, may serve as potent trigger signal for have been primed infection. These data frequently serious complications combined bacterial partially high production.