Transfer of single gene-containing long terminal repeats into the genome of mammalian cells by a retroviral vector carrying the cre gene and the loxP site.

摘要: Retroviral vectors contain viral cis-acting elements to achieve the packaging, reverse transcription, integration, and expression of retroviral genomic nucleic acid sequence. However, these are not useful in integrated provirus can be cause problems. We have developed a vector which eliminates majority elements. This vector, long terminal repeat (LTR) enhancer-deleted vectors, exploits Cre-lox recombination system P1 bacteriophage. The is neutral for eukaryotic cells. 32-nucleotide loxP site inserted within U3 3' LTR along with gene transduced (in place enhancers). Following LTR-mediated duplication, LTRs recombined by Cre enzyme. structure resulting host genome corresponds single (deleted enhancers) carrying copy transduced. If unit furnished after integration loxP-containing virus, efficiency absolute. between two LTRs, only proviral structures found following infection vector.