Detection and Typing of Human Papillomavirus in Archival Cervical Cancer Specimens by DNA Amplification With Consensus Primers

摘要: We developed a polymerase chain reaction DNA amplification system using two distinct consensus oligonucleotide primer sets for the improved detection and typing of broad spectrum human genital papillomavirus (HPV) sequences, including those novel viruses. The incorporates one set designed to amplify highly conserved L1 domain second within E6 gene. used this analyze 48 fixed, paraffin-embedded tissue sections (41 specimens from 33 cervical carcinomas, four normal tissues, several control tissues) presence HPV DNA. sequences were detected in all carcinoma samples none samples. Hybridization analyses showed that results obtained with schemes concurred completely. This approach allowed rapid confirmation may improve likelihood detecting wide variety HPVs.