Regional Distribution and Quantitative Measurement of the Phosphoinositidase C‐Linked Guanine Nucleotide Binding Proteins G1α and Gqα in Rat Brain

摘要: : Levels of the guanine nucleotide binding proteins G11α and Gqα, which produce receptor regulation phosphoinositidase C., were measured immunologically in 13 regions rat central nervous system. This was achieved by immunoblotting membranes from these with antisera (CQ series) that identify two polypeptides equally, following separation using sodium dodecyl sulphate-polyacrylamide gel electrophoresis conditions can resolve Gqα G11α. In all examined, more highly expressed than Ratios levels to varied between 5:1 2:1. Quantitative measurements each region obtained comparison known amounts purified liver E. coli recombinant Gqα. Areas included olfactory bulb (930 ng/ mg membrane protein), frontal cortex (700 ng/mg parietal occipital (670 caudate putamen (1,003 hippocampus (1,045 hypothalamus (790 cerebellum (950 protein). More modest observed thalamus (450 pituitary (480 optic chiasma (330 spinal cord (350 Gna evenly values ranging about 170 protein close 300 expressing high A third polypeptide could be identified CQ brain regions. The possibility this is α subunit G14 discussed.