Covalent Fluorescent Probes
作者： Richard P. Haugland
DOI: 10.1007/978-1-4615-9334-8_2
关键词: Biomolecule 、 Chemical bond 、 Fluorescence 、 Sulfonyl chloride 、 Dansyl chloride 、 Covalent bond 、 Ionic bonding 、 Photochemistry 、 Chemistry
摘要: In the application of fluorescence techniques to biomolecules it is frequently necessary introduce fluorophores with experimentally advantageous properties. These extrinsic fluorescent probes can be two kinds. Noncovalent form a reversible association biomolecule by combination hydrophobic, dipole—dipole, and ionic interactions. The covalent described in this chapter much stronger chemical bonds specific atoms on usually are essentially irreversibly bound. types complementary each has advantages, limitations, applications studies.
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