Proteins and glycoproteins of hamster kidney fibroblast (BHK21) plasma membranes and endoplasmic reticulum
作者： Carl G. Gahmberg
DOI: 10.1016/0005-2736(71)90085-X
关键词: Fibroblast 、 Biochemistry 、 Chemistry 、 Polyacrylamide gel electrophoresis 、 Membrane 、 Endoplasmic reticulum 、 Glycoprotein 、 Fucose 、 Hamster 、 Glucosamine 、 Biophysics 、 Cell biology
摘要: Abstract 1. Hamster fibroblast plasma membranes and endoplasmic reticulum were solubilized by sodium dodecyl sulphate 2-mercaptoethanol treatment studied polyacrylamide gel electrophoresis in the presence of sulphate. The electrophoretic patterns different. amino acid compositions three major membrane protein bands determined found to differ significantly. Both contained a fast moving component low apparent molecular weight ( 2. When cells labelled with glucosamine or fucose labels 9–12 times more concentrated than homogenate. weights glycoproteins electrophoresis.
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sci-hub.se 参考文章(50)
Janos Molnar, Gordana Markovic, Helen Chao, Zelma Molnar, Glycoproteins of Ehrlich ascites carcinoma cells Archives of Biochemistry and Biophysics. ,vol. 134, pp. 524- 532 ,(1969) , 10.1016/0003-9861(69)90314-2
D.B. Thomas, Richard J. Winzler, Structural Studies on Human Erythrocyte Glycoproteins ALKALI-LABILE OLIGOSACCHARIDES Journal of Biological Chemistry. ,vol. 244, pp. 5943- 5946 ,(1969) , 10.1016/S0021-9258(18)63563-X
David Schubert, Immunoglobulin biosynthesis: IV. Carbohydrate attachment to immunoglobulin subunits Journal of Molecular Biology. ,vol. 51, pp. 287- 301 ,(1970) , 10.1016/0022-2836(70)90143-9
Harry Schachter, Inderjit Jabbal, Roger L. Hudgin, L. Pinteric, Edward J. McGuire, Saul Roseman, Intracellular Localization of Liver Sugar Nucleotide Glycoprotein Glycosyltransferases in a Golgi-rich Fraction Journal of Biological Chemistry. ,vol. 245, pp. 1090- 1100 ,(1970) , 10.1016/S0021-9258(18)63293-4
B. de Crombrugghe, R. Pitt-Rivers, Harold Edelhoch, The Properties of Thyroglobulin XI. THE REDUCTION OF THE DISULFIDE BONDS Journal of Biological Chemistry. ,vol. 241, pp. 2766- 2773 ,(1966) , 10.1016/S0021-9258(18)96530-0
Giorgio Lenaz, Norman F. Haard, H.Israel Silman, David E. Green, Studies on mitochondrial structural protein Archives of Biochemistry and Biophysics. ,vol. 128, pp. 293- 303 ,(1968) , 10.1016/0003-9861(68)90035-0
G. Ross Lawford, Harry Schachter, Biosynthesis of Glycoprotein by Liver THE INCORPORATION IN VIVO OF 14C-GLUCOSAMINE INTO PROTEIN-BOUND HEXOSAMINE AND SIALIC ACID OF RAT LIVER SUBCELLULAR FRACTIONS Journal of Biological Chemistry. ,vol. 241, pp. 5408- 5418 ,(1966) , 10.1016/S0021-9258(18)96445-8
S A Rosenberg, G Guidotti, The Protein of Human Erythrocyte Membranes. I. Preparation, Solubilization, and Partial Characterization Journal of Biological Chemistry. ,vol. 243, pp. 1985- 1992 ,(1968) , 10.1016/S0021-9258(18)93538-6
Wayne N. Yunghans, T.W. Keenan, D.James Morré, Isolation of Golgi apparatus from rat liver: III. Lipid and Protein Composition Experimental and Molecular Pathology. ,vol. 12, pp. 36- 45 ,(1970) , 10.1016/0014-4800(70)90073-0
K Weber, M Osborn, The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis Journal of Biological Chemistry. ,vol. 244, pp. 4406- 4412 ,(1969) , 10.1016/S0021-9258(18)94333-4