The effects of hydrostatic pressure on signal transduction in brain membranes of deep-sea fishes of the genus Coryphaenoides

摘要: To investigate the effects of increased hydrostatic pressure on transmembrane signaling in deep-living marine species, A1 adenosine receptor − inhibitory G protein (Gi) adenylyl cyclase complex was examined brain membrane preparations from four teleost fish species deep-sea family Macrouridae. The combined depth ranges adults these Coryphaenoides armatus, C. cinereus, filifer, pectoralis, span several hundred meters to 5400 m. Basal activity, determined at 5 °C, inhibited by all species. At highest tested, 476 atm, activity 60 70% relative atmospheric values. Pressure inhibition did not result denaturation or loss components due pressure-induced shedding. Despite pressure-inhibition basal responsiveness modulation N6-cyclopentyladenosine, an agonist, retained elevated pressures. Because accumulation second messenger cAMP depends summation modulatory inputs, results indicate that is insensitive changes.