Electrophysiological phenotyping in genetically engineered mice

摘要: Advances in transgene and gene targeting technology have enabled sophisticated manipulation of the mouse genome, providing important insights into molecular mechanisms underlying cardiac conduction, arrhythmogenesis, sudden death. The is currently principal mammalian model for studying biological processes, particularly related to pathophysiology. Murine models been engineered harboring mutations leading inherited structural electrical disorders heart due transcription factor mutations, connexin protein defects, G ion channelopathies. These lead phenotypes reminiscent human clinical disease states including congenital cardiomyopathies, long-QT syndrome, creating electrophysiological disease. Functional analyses resultant require appropriate experimental methodology. This paper reviews current vivo murine electrophysiology study techniques genetic pertinent arrhythmia disorders.