Protective effects of kaempferol against endothelial damage by an improvement in nitric oxide production and a decrease in asymmetric dimethylarginine level.
作者： Hong-Bo Xiao , Jun-Fang , Xiang-Yang Lu , Xiao-jun Chen , Chao-Tan
DOI: 10.1016/J.EJPHAR.2009.06.022
关键词: Asymmetric dimethylarginine 、 Nitric oxide synthase 、 Nitric oxide 、 Endocrinology 、 Human umbilical vein endothelial cell 、 Endothelial dysfunction 、 Internal medicine 、 Chemistry 、 Biochemistry 、 Kaempferol 、 Endothelial NOS 、 Endothelium
摘要: Previous investigations have shown that asymmetric dimethylarginine (ADMA) inhibits nitric oxide (NO) synthases (NOS) and ADMA is a risk factor for endothelial dysfunction. The objective of this study was to investigate the protective effect kaempferol, naturally occurring flavonoid antioxidant agent, against damage mechanisms involved. experiments were performed in aorta plasma from C57BL/6J control apolipoprotein E-deficient (ApoE(-/-)) mice treated or not with kaempferol (50 100mg/kg, intragastrically) 4 weeks, human umbilical vein cells (HUVECs) pretreated (1, 3 10 microM) 1h exposed lysophosphatidylcholine (LPC) (10 microg/mL) 24h. Kaempferol treatment improved endothelium-dependent vasorelaxation, increased maximal relaxation value, decreased half-maximum effective concentration concomitantly an increase concentration, decrease malondialdehyde (MDA) concentrations, expression aortic NOS (eNOS) as well dimethylaminohydrolase II (DDAH II) ApoE(-/-) mice. In addition, LPC caused reduction NO production, eNOS DDAH HUVECs, abolished by kaempferol. Treatment also significantly reactive oxygen species production HUVECs. present results suggest may be associated improvement level.
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