Determination of free and bound 3-chloropropane-1,2-diol by gas chromatography with mass spectrometric detection using deuterated 3-chloropropane-1,2-diol as internal standard

摘要: An improved routine, simple and sensitive method is presented for the determination of free bound 3-chloropropane-1,2-diol (3-MCPD) in different foods using capillary gas chromatography with mass spectrometric detection deuterated 3-MCPD as internal standard. The optimised was linear within working calibration standard concentrations range 0.009-1.3 mg per 1 kg sample. LOD LOQ were 0.003 μg/kg 0.009 μg/kg, respectively. Validation carried out by analysing standards 3-MCPD, acid-HVP, roasted coffee samples, same samples spiked 3-MCPD. Repeatability (expressed RSD) 1.0-4,2%, average spike recoveries 99.1-99.5% (RSD = 0.8-1.4%), esters higher fatty acids isolated fat, fat subjected to methanolysis generated quantified method. determined be 1.1 mg/kg lipids 3.3 lipids, Using method, 20 retail food products analysed their All contained at 9.6-83 0.4-7.0%). level varied between 2.4 RSD 0.3-2.4%.