The Fps1p aquaglyceroporin facilitates the use of small aliphatic amides as a nitrogen source by amidase-expressing yeasts.

摘要: Saccharomyces cerevisiae acquires a resistance to high, toxic levels of acetic acid by destabilizing Fps1p, the plasma membrane aquaglyceroporin through which this – in its undissociated state enters cell. In study, Fps1p loss was shown confer resistances acid, acrolein and allyl alcohol, not just S. but also osmotolerant spoilage yeast Zygosaccharomyces rouxii. However, Z. rouxii, severely compromised use acetamide several other small amides as sources nitrogen, an indication that these enter cells passive diffusion pore. cannot grow on acetamide, conferred with ability nitrogen heterologous expression rouxii ORF (ZrAMD1) protein sequence identity amdS-encoded amidase Aspergillus nidulans. This capacity ZrAMD1-expressing assimilate amide Fps1p. ZrAMD1 appears encode major Zramd1Δ deletant mutant had, like Zrfps1Δ deletant, lost nitrogen.