Molecular phylogeny and phylogeography of Lupinus ( Leguminosae ) inferred from nucleotide sequences of the rbc L gene and ITS 1 + 2 regions of rDNA
作者： Ernst K�ss , Michael Wink
DOI: 10.1007/BF00985439
关键词: Phylogenetic tree 、 Phylogeography 、 Molecular phylogenetics 、 Biology 、 Monophyly 、 Maximum parsimony 、 Botany 、 Evolutionary biology 、 Sister group 、 Old World 、 Clade
摘要: Total DNA was extracted from 55 species of theLeguminosae (including 29 ofLupinus). The chloroplast generbcL and the ITS 1 + 2 regions nuclear RNA genes were amplified by polymerase chain reaction (PCR) sequenced directly. sequences obtained evaluated with character state (Maximum Parsimony) distance methods (Neighbour Joining). Phylogenetic trees both data sets are mostly congruent.Genisteae andCrotalarieae sister groups share ancestry theThermopsideae/Podalyrieae. genusLupinus, which forms a monophyletic clade within theGenisteae, shows distinct Old-New World disjunction appears to be divided into several more or less groups: (1) eastern part South America. (2) homogeneous rough-seeded group (Scabrispermae) Old is well distinguished smooth-seeded (Malacospermae). (3) Within rather heterogeneous lupins smaller subgroup withL. angustifolius, L. hispanicus andL. luteus recognized. (4) Also separated North American western distribution. Genetic distances imply that genusLupinus evolved during last 12–14 million years, ruling out hypothesis present can interpreted as result continental drift. genetic suggest an origin in independant colonisation Eastern parts America opposed Western
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