A method of endotracheal intubation and pulmonary functional assessment for repeated studies in mice

摘要: The ability to successfully intubate the trachea of mice and control their ventilation is important for longitudinal studies requiring recovery from anesthesia repeated pulmonary function measurements or other evaluations, such as use radiological imaging (e.g., computed tomography magnetic resonance imaging). We describe a method rapid intubation mice, with subsequent at baseline after an agonist challenge. simply constructed metal blade used laryngoscope facilitate oropharyngeal exposure, transillumination neck visualization through oropharynx, readily available polyethylene tubing trachea, simple solenoid ventilator maintain physiological assess respiratory resistance compliance. Brief infusions acetylcholine needle into jugular vein are responsiveness airway smooth muscle.