Promoter Region of the Rat m4 Muscarinic Acetylcholine Receptor Gene Contains a Cell Type-specific Silencer Element

摘要: We describe here the characterization of rat m4 muscarinic acetylcholine receptor gene and identification its regulatory region. Two 5′-noncoding exons are located approximately 5 kilobases upstream from coding exon, at least two alternatively spliced variants mRNA expressed in neuronal cell line PC12D. There transcription initiation sites. The promoter region is GC-rich, contains no TATA-box, but has potential CAAT boxes several putative binding sites for factors Sp1 AP-2. assessed activity functionally transient expression assays using luciferase as a reporter. proximal 435-base pair (bp) sequence 5′-flanking produced both m4-expressing lines (PC12D NG108-15) non-neuronal (L6 3Y1B). A longer fragment containing an additional 638-bp only lines. These data suggest that 435-bp constitutive farther type-specific silencer element. consensus neural-restrictive element found within this segment.