Microarray analysis of gene expression in seeds of Brassica napus planted in Nanjing (altitude: 8.9 m), Xining (altitude: 2261.2 m) and Lhasa (altitude: 3658 m) with different oil content

摘要: The regulation of seed oil synthesis in rapeseed is largely unknown. In this study, Arabidopsis microarray was used to analyze the gene differential expression immature seeds 30 days after flowering a high Brassica napus, H105, whose content 46.04 +/- 1.42, 53.94 1.35 and 53.09 1.35% when planted Nanjing (altitude: 8.9 m), Xining 2261.2 m) Lhasa 3658 respectively. Transcript levels 363 genes 421 were altered twofold or more for H105 compared that Nanjing, Together, there 53 common up-regulated 42 down-regulated transcripts shared by Nanjing. Some important genes, such as sucrose synthase, pyruvate kinase 6-phosphogluconate dehydrogenase which related sugar metabolism identified higher H105. These results revealed expressional disciplinarian correlative provided information molecular genetic mechanism difference rapeseed. addition, these could be suitable targets improvement content.