Single Nucleotide Polymorphism (SNP) Markers for Allele Quantification in Lolium (Poaceae): Development and First Applications
作者： Tatjana Sretenovic Rajicic , Thomas Lübberstedt , Louise Bach Jensen , Uwe Scholz , W. Eberhard Weber
DOI: 10.1007/978-3-319-08714-6_13
关键词: Genetics 、 Germplasm 、 Single-nucleotide polymorphism 、 Pyrosequencing 、 Lolium perenne 、 Biology 、 Population 、 Lolium 、 Polymorphism (computer science) 、 SNP
摘要: Lolium grasses are widely distributed and used, their economic importance is significant. We report the development of single nucleotide polymorphism (SNP) markers that were derived from barley EST data suitable for allele quantification (AQ) via pyrosequencing (PSQ). Polymorphism information content (PIC) values 22 developed ranged between 0.23 0.93 (average PIC value 0.80). The used to characterize a collection 2829 accessions. Eleven SNP could be mapped in perenne VrnA mapping population. This first on fingerprinting comprehensive genebank by alleles. clearly distinguish L. multiflorum will prove as very useful management germplasm collections.
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