Cloning by complementation and related processes
作者： John J. Colicelli , Michael H. Wigler
DOI:
关键词: Cloning 、 Complementation 、 RNA 、 Genetics 、 Function (biology) 、 Cell biology 、 Metabolic pathway 、 Biology 、 Gene 、 DNA sequencing 、 Phenotype
摘要: Disclosed are methods for detecting mammalian genes encoding proteins which can function in microorganisms, particularly yeast, to modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration characteristic the microorganism. also DNA sequences cloned by above method, as well polypeptide products of expression procaryotic eucaryotic host cells and antibody substances specifically immunoreactive said products. More specifically, present invention relates cloning encode complement biochemical pathway cAMP participates is controlled, directly indirectly, RAS-related protein, (RNA, proteins) encoded this manner, antibodies bind proteins.
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