The T lymphocyte response to cytochrome c--II. Molecular characterization of a pigeon cytochrome c determinant recognized by proliferating T lymphocytes of the B10.A mouse.

摘要: The molecular specificity of the 1 lymphocyte proliferative response to pigeon cytochrome c was investigated by testing numerous other species' cytochromes and their cyanogen bromide cleavage fragments T Ivmnhocytes B10. A mice immunized protein. Amino acid residues having a major effect on were those located at positions 100, 103 104. immunodominant antigenic had been previously localized carboxyl-terminal region protein, this localization confirmed in current study responses seen C-terminal peptide hippopotamus tobacco hornworm moth c. Characterization an unexpected cross-stimulation with tuna led finding that serine could completely substitute for glutamine position discovery second c, from screw worm fly, elicit heteroclitic cell response. From our comparative studies different it appeared both uncharged residue lysine or 104, required full stimulation. However, undoubtedly play role structure determinant, such as spatially-close isoleucine 3, which found be responsible stimulatory activity certain fragments. This point highlighted failure synthesize small stimulate No either surface-simulated peptides extending up eight amino acids length containing identified determinant. apparent requirement large order achieve antigenicity suggests formation determinant requires some degree secondary tertiary important exists molecule, between 81 96, perhaps constitutes site macrophage binds.