Collagenase-3 (MMP-13) Expression in Chondrosarcoma Cells and Its Regulation by Basic Fibroblast Growth Factor

摘要: Human collagenase-3 (MMP-13) is a member of the matrix metalloproteinase family enzymes that was originally identified in breast carcinomas and subsequently detected during fetal ossification arthritic processes. In this work, we have found produced by HCS-2/8 human chondrosarcoma cells. An analysis ability different cytokines growth factors to induce expression these cells revealed basic fibroblast factor (bFGF or FGF-2) strongly up-regulated gene. By contrast, other factors, including interleukin-1β transforming factor-β, previously cell types, did not exhibit any effect on gene Further bFGF-induced its time dose dependent, but independent de novo synthesis proteins. Western blot up-regulatory bFGF also reflected at protein level as demonstrated increase immunoreactive conditioned medium treated with bFGF. Immunohistochemical presence series 8 benign 16 malignant cartilage-forming neoplasms all analyzed chondrosarcomas stained positively for collagenase-3, whereas only 2 lesions protease. addition, finding chondrosarcomas, together above vitro studies cells, suggest may be an vivo modulator tumors. These results extend pattern tumor types produce up-regulation contribute progression chondrosarcomas.