Antigen-Induced Expression of CD203c on Basophils Predicts IgE-mediated Wheat Allergy

摘要: ABSTRACT Background For in vitro diagnosis of wheat allergy, specific IgE to is known be a poor predictive marker. Oral food challenge, the gold standard for diagnosis, accompanied by risk severe induced reactions. Reliable tests are needed developed safe indication oral challenge. Objective We examined utility basophil activation marker, CD203c, IgE-mediated allergy. Methods Fifty-eight children with suspected allergy positive CAP-FEIA were enrolled. On 70 occasions, clinical distinction between patients (WA) and tolerant (TW) was made means an challenge test or recent history immediate allergic reactions tolerance after ingestion wheat. Twelve replicate evaluations performed 9 over more than 6-month interval. Thirty two on 43 occasions diagnosed WA 27 confirmed TW. One patient had both diagnoses 18 months apart. Peripheral blood incubated fractionated extracts, purified native omega-5 gliadin (nOG5) recombinant (rOG5). Expression CD203c basophils then analyzed flow cytometry using commercial kit. Results All proteins concentration-dependent enhancement expression WA, but did not The analysis receiver operating characteristics (ROC) showed that nOG5-induced high % values provided best power discriminating TW, sensitivity 85.0% specificity 77.0% at cut-off level 14.4%. AUC nOG5 significantly higher conventional CAP-FEIA, 0.89 0.73, respectively (p Conclusions Measurement nOG-induced useful children.