Fentanyl inhibits progression of human gastric cancer MGC-803 cells by NF-kappaB downregulation and PTEN upregulation in vitro.

摘要: Fentanyl is used as an analgesic to treat pain in a variety of patients with cancer. Moreover, fentanyl may affect tumor growth many cell lines. To gain better insight into the interaction between and tumor, we investigated effects on gastric carcinoma cells expression some apoptosis-related genes including NF-kappaB PTEN. A human cancer line MGC-803 was used. The viability proliferation were detected by MTT assay colony formation assay. cycle progression apoptosis assessed flow cytometry ultrastructure examined transmission electron microscope. migration wound healing PTEN evaluated semiquantitative RT-PCR Western blot. Our data showed that could inhibit made arrest at G2/M phase. Compared control cells, incubated also had higher apoptotic rate. lead morphological changes reduce motility cells. downregulate upregulate PTEN, which might be mechanism inhibiting vitro.