Quantification of peripheral whole blood, cell-free plasma and exosome encapsulated mitochondrial DNA copy numbers in patients with atrial fibrillation.
作者： Beáta Soltész , Réka Urbancsek , Ondrej Pös , Orsolya Hajas , Ildikó Noémi Forgács
DOI: 10.1016/J.JBIOTEC.2019.04.018
关键词: DNA 、 Andrology 、 Biology 、 Microvesicles 、 Primer (molecular biology) 、 Whole blood 、 Mitochondrial DNA 、 Oxidative stress 、 Human mitochondrial genetics 、 Exosome
摘要: Abstract Mitochondrial DNA (mtDNA) copy number changes have been associated with various diseases. Several studies showed that mtDNA content in peripheral blood was oxidative stress and cardiovascular disease. Atrial fibrillation (AF) is one of the severe We aimed to determine numbers blood, cell-free plasma exosomes AF patients healthy controls. Peripheral drawn from 60 72 isolated EDTA plasma. Exosomes were then exosome encapsulated (exoDNA) extracted. Quantitative-real-time PCR performed Human Monitoring Primer Set. Statistical analysis data performed. found statistically significant difference whole samples controls’ (44.4 ± 18.0, 27.2 ± 30.1, 11.5 ± 8.7), patients’ group (43.4 ± 13.6, 26.2 ± 26.4, 14.5 ± 12.3). However there no between two study groups either exosomes, even different sexes ages. highest followed by exosomes. did not find differences controls, neither age nor gender had effect on number. According our results differ patients.
sci-hub.se 参考文章(38)
YAU-HUEI WEI, CHING-YOU LU, HSIN-CHEN LEE, CHENG-YOONG PANG, YI-SHING MA, Oxidative Damage and Mutation to Mitochondrial DNA and Age-dependent Decline of Mitochondrial Respiratory Functiona Annals of the New York Academy of Sciences. ,vol. 854, pp. 155- 170 ,(1998) , 10.1111/J.1749-6632.1998.TB09899.X
H.-W. Park, Y. Ahn, M.-H. Jeong, J.-G. Cho, J.-C. Park, J.-C. Kang, M.-G. Shin, J.-H. Shin, S.-P. Suh, D.-W. Ryang, N.-H. Kim, J.-B. Choi, H.-R. Kim, Chronic atrial fibrillation associated with somatic mitochondrial DNA mutations in human atrial tissue. Journal of Clinical Pathology. ,vol. 60, pp. 948- 950 ,(2006) , 10.1136/JCP.2007.047134
Jodi Nunnari, Anu Suomalainen, Mitochondria: In Sickness and in Health Cell. ,vol. 148, pp. 1145- 1159 ,(2012) , 10.1016/J.CELL.2012.02.035
Shuying Chen, Xuejian Xie, Yujing Wang, Yan Gao, Xiaoli Xie, Jing Yang, Jixian Ye, Association between leukocyte mitochondrial DNA content and risk of coronary heart disease: a case-control study. Atherosclerosis. ,vol. 237, pp. 220- 226 ,(2014) , 10.1016/J.ATHEROSCLEROSIS.2014.08.051
Sumeet S Chugh, Rasmus Havmoeller, Kumar Narayanan, David Singh, Michiel Rienstra, Emelia J Benjamin, Richard F Gillum, Young-Hoon Kim, John H McAnulty, Zhi-Jie Zheng, Mohammad H Forouzanfar, Mohsen Naghavi, George A Mensah, Majid Ezzati, Christopher JL Murray, None, Worldwide Epidemiology of Atrial Fibrillation: A Global Burden of Disease 2010 Study Circulation. ,vol. 129, pp. 837- 847 ,(2014) , 10.1161/CIRCULATIONAHA.113.005119
Christian T Ruff, Robert P Giugliano, Eugene Braunwald, Elaine B Hoffman, Naveen Deenadayalu, Michael D Ezekowitz, A John Camm, Jeffrey I Weitz, Basil S Lewis, Alexander Parkhomenko, Takeshi Yamashita, Elliott M Antman, Comparison of the efficacy and safety of new oral anticoagulants with warfarin in patients with atrial fibrillation: a meta-analysis of randomised trials The Lancet. ,vol. 383, pp. 955- 962 ,(2014) , 10.1016/S0140-6736(13)62343-0
Y Hatefi, The mitochondrial electron transport and oxidative phosphorylation system Annual Review of Biochemistry. ,vol. 54, pp. 1015- 1069 ,(1985) , 10.1146/ANNUREV.BI.54.070185.005055
Alberto Cresti, Silva Severi, Günter Breithardt, Francesco De Sensi, Tom De Potter, Atrial fibrillation in patients with diabetes: molecular mechanisms and therapeutic perspectives. Cardiovascular diagnosis and therapy. ,vol. 5, pp. 364- 373 ,(2015) , 10.3978/J.ISSN.2223-3652.2015.06.03
N. P. Sudakov, T. P. Popkova, A. I. Katyshev, O. A. Goldberg, S. B. Nikiforov, B. G. Pushkarev, I. V. Klimenkov, S. A. Lepekhova, K. A. Apartsin, G. A. Nevinsky, Yu. M. Konstantinov, Level of blood cell-free circulating mitochondrial DNA as a novel biomarker of acute myocardial ischemia Biochemistry. ,vol. 80, pp. 1387- 1392 ,(2015) , 10.1134/S000629791510020X
William J. Hucker, Harsimran Saini, Steven A. Lubitz, Patrick T. Ellinor, Atrial Fibrillation Genetics: Is There a Practical Clinical Value Now or in the Future? Canadian Journal of Cardiology. ,vol. 32, pp. 1300- 1305 ,(2016) , 10.1016/J.CJCA.2016.02.032