Proteome analysis of human visceral and subcutaneous adipocytes identifies depot-specific heterogeneity at metabolic control points

摘要: Abstract Adipose tissue is a primary regulator of energy balance and metabolism. The distribution adipose depots clinical interest because the accumulation upper-body subcutaneous (ASAT) visceral (VAT) associated with cardiometabolic diseases, whereas lower-body gluteal-femoral (GFAT) appears to be protective. There heterogeneity in morphology metabolism adipocytes obtained from different regions body, but detailed knowledge constituent proteins each depot lacking. Here, we determined human adipocyte proteome ASAT, VAT GFAT using high-resolution SWATH mass spectrometry proteomics. We quantified 4220 adipocytes, 2329 were expressed all three depots. Comparative analysis revealed significant differences between (6 8% when comparing vs. ASAT GFAT, 3% GFAT), marked that regulate metabolic functions. was overrepresented glycolysis, lipogenesis, oxidative stress mitochondrial dysfunction. predicted activation PPARα, fatty acid BCAA oxidation, enhanced TCA cycle flux phosphorylation, which supported by metabolomic data same patient donors. Together, this proteomic provides an important resource novel insights enhance understanding regional humans.