INTERACTIONS BETWEEN pH AND MALIC ACID CONCENTRATION ON THE INACTIVATION OF LISTERIA MONOCYTOGENES

摘要: The effects and interactions of malic acid concentration pH on the inactivation kinetics a three strain mixture Listeria monocytogenes was studied in brain heart infusion broth (BHI). medium supplemented with monosodium malate to achieve levels 3.0, 3.5, 4.0, 4.5, 5. 0, 5.5, 6. 6.5 or 7.0 conjunction concentrations 0.0, 0.1, 0. 5, 1.0 2. 0 M. Duplicate 20-mL portions each pH/malate level were inoculated (ca. 10 8 cfu/mL), stored aerobically at 28C, assayed periodically for viable counts by plating BHI agar. Survivor curves generated fitting data linear model that includes lag term used calculate D-values times 4-D . Inactivation rates dependent both concentration. At higher levels, appeared provide some degree protection compared control cultures where adjusted HCl. lower values concentrations, concentration-dependent anion effect observed. results indicate is relatively benign organic acid. Its antimicrobial characteristics are similar those citric substantially less bactericidal than lactic acetic acids.