Caged compounds for multichromic optical interrogation of neural systems

摘要: Caged compounds are widely used by neurophysiologists to study many aspects of cellular signaling in glia and neurons. Biologically inert before irradiation, they can be loaded into cells via patch pipette or topically applied situ a defined concentration; photolysis releases the caged compound very rapid spatially way. As exogenous optical probes, include not only natural products such neurotransmitters, calcium IP3 but non-natural as fluorophores, drugs antibodies. In this Technical Spotlight we provide short introduction uncaging technique discussing nitroaromatic caging chromophores most experiments [e.g. α-carboxy-ortho-nitrobenyl (CNB), dimethoxynitrobenzyl (DMNB), 4-methoxy-7-nitroindolinyl (MNI) 4-carboxymethoxy-7-nitroindolinyl (CDNI)]. We show that recently developed [rutheniumbipyridial (RuBi) 7-diethylaminocoumarin (DEAC)450] photolyzed with blue light (~ 430–480 nm range) combined traditional enable two-color probing neuronal function. For example, one-photon either RuBi-GABA DEAC450-GABA 473-nm laser is facile, block nonlinear currents (dendritic spikes action potentials) evoked two-photon CDNI-Glu at 720 nm. also DEAC450-Glu CDNI-GABA 900 720 nm, respectively, fire potentials. Our illustrate have taken out ‘monochrome era’, which it has existed since 1978, so multichromic interrogation function single-synapse precision.