Evaluating variation in human gut microbiota profiles due to DNA extraction method and inter-subject differences

作者: Brett Wagner Mackenzie , David W. Waite , Michael W. Taylor

DOI: 10.3389/FMICB.2015.00130

关键词: DNAHuman Microbiome ProjectNucleic acidGeneticsBiologyMicrobial population biologyMicrobial DNADNA extractionPlasmid preparationGene

摘要: … human gut contains dense and diverse microbial communities which have profound influences on human … of high quality microbial nucleic acids from human fecal samples, as well as …

参考文章(53)
Zhongtang Yu, Mark Morrison, Improved extraction of PCR-quality community DNA from digesta and fecal samples. BioTechniques. ,vol. 36, pp. 808- 812 ,(2004) , 10.2144/04365ST04
Christian L. Lauber, Nicholas Zhou, Jeffrey I. Gordon, Rob Knight, Noah Fierer, Effect of storage conditions on the assessment of bacterial community structure in soil and human-associated samples Fems Microbiology Letters. ,vol. 307, pp. 80- 86 ,(2010) , 10.1111/J.1574-6968.2010.01965.X
Gary D Wu, Jun Chen, Christian Hoffmann, Kyle Bittinger, Ying-Yu Chen, Sue A Keilbaugh, Meenakshi Bewtra, Dan Knights, William A Walters, Rob Knight, Rohini Sinha, Erin Gilroy, Kernika Gupta, Robert Baldassano, Lisa Nessel, Hongzhe Li, Frederic D Bushman, James D Lewis, Linking Long-Term Dietary Patterns with Gut Microbial Enterotypes Science. ,vol. 334, pp. 105- 108 ,(2011) , 10.1126/SCIENCE.1208344
Luke K. Ursell, Jose C. Clemente, Jai Ram Rideout, Dirk Gevers, J. Gregory Caporaso, Rob Knight, The interpersonal and intrapersonal diversity of human-associated microbiota in key body sites The Journal of Allergy and Clinical Immunology. ,vol. 129, pp. 1204- 1208 ,(2012) , 10.1016/J.JACI.2012.03.010
Alexandra L. McOrist, Michelle Jackson, Anthony R. Bird, A comparison of five methods for extraction of bacterial DNA from human faecal samples. Journal of Microbiological Methods. ,vol. 50, pp. 131- 139 ,(2002) , 10.1016/S0167-7012(02)00018-0
Shantelle Claassen, Elloise du Toit, Mamadou Kaba, Clinton Moodley, Heather J. Zar, Mark P. Nicol, A comparison of the efficiency of five different commercial DNA extraction kits for extraction of DNA from faecal samples Journal of Microbiological Methods. ,vol. 94, pp. 103- 110 ,(2013) , 10.1016/J.MIMET.2013.05.008
T. Z. DeSantis, P. Hugenholtz, N. Larsen, M. Rojas, E. L. Brodie, K. Keller, T. Huber, D. Dalevi, P. Hu, G. L. Andersen, Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB Applied and Environmental Microbiology. ,vol. 72, pp. 5069- 5072 ,(2006) , 10.1128/AEM.03006-05
E. K. Costello, C. L. Lauber, M. Hamady, N. Fierer, J. I. Gordon, R. Knight, Bacterial Community Variation in Human Body Habitats Across Space and Time Science. ,vol. 326, pp. 1694- 1697 ,(2009) , 10.1126/SCIENCE.1177486
Gemma Henderson, Faith Cox, Sandra Kittelmann, Vahideh Heidarian Miri, Michael Zethof, Samantha J. Noel, Garry C. Waghorn, Peter H. Janssen, Effect of DNA Extraction Methods and Sampling Techniques on the Apparent Structure of Cow and Sheep Rumen Microbial Communities PLoS ONE. ,vol. 8, pp. e74787- ,(2013) , 10.1371/JOURNAL.PONE.0074787
Nicholas A Kennedy, Alan W Walker, Susan H Berry, Sylvia H Duncan, Freda M Farquarson, Petra Louis, John M Thomson, Jack Satsangi, Harry J Flint, Julian Parkhill, Charlie W Lees, Georgina L Hold, None, The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene Sequencing PLoS ONE. ,vol. 9, pp. e88982- ,(2014) , 10.1371/JOURNAL.PONE.0088982