PURIFICATION AND CHARACTERIZATION OF THE SELENATE REDUCTASE FROM THAUERA SELENATIS
作者： Imke Schröder , Sabine Rech , Torsten Krafft , Joan M. Macy
关键词: Enzyme 、 Periplasmic space 、 Biochemistry 、 Thauera selenatis 、 Selenium 、 Selenate 、 Chemistry 、 Cofactor 、 Chlorate 、 Selenate reductase
摘要: Thauera selenatis is one of two isolated bacterial species that can obtain energy by respiring anaerobically with selenate as the terminal electron acceptor. The reduction to selenite catalyzed a reductase, previously shown be located in periplasmic space cell. This study describes purification enzyme from T. grown selenate. trimeric alphabetagamma complex an apparent Mr 180,000. alpha, beta, and gamma subunits are 96 kDa, 40 23 respectively, size. reductase contains molybdenum, iron, acid-labile sulfur prosthetic group constituents. UV-visible absorption spectroscopy also revealed presence cytochrome b per complex. Km for was determined 16 microM, Vmax micromol/min/mg protein. specific selenate; nitrate, nitrite, chlorate, sulfate were not reduced at detectable rates. These studies constitute first description which represents new class enzymes. significance this relation cell growth generation discussed.
sci-hub.se 参考文章(30)
Tsoo E. King, Roy O. Morris, [98] Determination of acid-labile sulfide and sulfhydryl groups Methods in Enzymology. ,vol. 10, pp. 634- 641 ,(1967) , 10.1016/0076-6879(67)10105-5
Lucile Smith, Bacterial cytochromes and their spectral characterization. Methods in Enzymology. ,vol. 53, pp. 202- 212 ,(1978) , 10.1016/S0076-6879(78)53025-5
Philip E. Brumby, Vincent Massey, [73] Determination of nonheme iron, total iron, and copper Methods in Enzymology. ,vol. 10, pp. 463- 474 ,(1967) , 10.1016/0076-6879(67)10078-5
L. Lortie, W. D. Gould, S. Rajan, R. G. L. McCready, K.-J. Cheng, Reduction of Selenate and Selenite to Elemental Selenium by a Pseudomonas stutzeri Isolate. Applied and Environmental Microbiology. ,vol. 58, pp. 4042- 4044 ,(1992) , 10.1128/AEM.58.12.4042-4044.1992
Ronald S. Oremland, James T. Hollibaugh, Ann S. Maest, Theresa S. Presser, Laurence G. Miller, Charles W. Culbertson, Selenate Reduction to Elemental Selenium by Anaerobic Bacteria in Sediments and Culture: Biogeochemical Significance of a Novel, Sulfate-Independent Respiration Applied and Environmental Microbiology. ,vol. 55, pp. 2333- 2343 ,(1989) , 10.1128/AEM.55.9.2333-2343.1989
S A Rech, J M Macy, The terminal reductases for selenate and nitrate respiration in Thauera selenatis are two distinct enzymes. Journal of Bacteriology. ,vol. 174, pp. 7316- 7320 ,(1992) , 10.1128/JB.174.22.7316-7320.1992
Nisan A. Steinberg, Ronald S. Oremland, Dissimilatory Selenate Reduction Potentials in a Diversity of Sediment Types Applied and Environmental Microbiology. ,vol. 56, pp. 3550- 3557 ,(1990) , 10.1128/AEM.56.11.3550-3557.1990
I Schröder, C D Wolin, R Cavicchioli, R P Gunsalus, Phosphorylation and dephosphorylation of the NarQ, NarX, and NarL proteins of the nitrate-dependent two-component regulatory system of Escherichia coli. Journal of Bacteriology. ,vol. 176, pp. 4985- 4992 ,(1994) , 10.1128/JB.176.16.4985-4992.1994
R A Siddiqui, U Warnecke-Eberz, A Hengsberger, B Schneider, S Kostka, B Friedrich, Structure and function of a periplasmic nitrate reductase in Alcaligenes eutrophus H16. Journal of Bacteriology. ,vol. 175, pp. 5867- 5876 ,(1993) , 10.1128/JB.175.18.5867-5876.1993
R S Oremland, N A Steinberg, T S Presser, L G Miller, In situ bacterial selenate reduction in the agricultural drainage systems of western Nevada Applied and Environmental Microbiology. ,vol. 57, pp. 615- 617 ,(1991) , 10.1128/AEM.57.2.615-617.1991