Nanoparticle-Protein Interaction Studies Using Spectroscopic and Biophysical Methods

摘要: The interaction of nanoparticles with biological interfaces has been studied extensively, where it found that proteins form the most abundant class biomolecules interacting nanoparticles. Also, due to huge potential they offer are being constantly scrutinized for their role in various aspects biomedical, therapeutic and biotechnological applications. As a matter fact, exposure such particulate living systems is increasing exponentially, raising serious concerns related safety. toxicity may arise interact any system, even crossing protected blood brain barrier. In addition, can induce conformational changes protein, alter fluidity membranes, release reactive oxygen species (ROS) cause severe damage DNA. Since luring, deleterious manifestations need be alleviated order tap resource provide. This challenge, since as complex structure proteins. Here, we have developed novel synthetic approach surface modification one biocompatible magnetite more modifying agent, poly(ethylene) glycol. stability efficiency new procedure extensively analysed. different protein taken account. It was observed bare affect differently than modified ones. systems, redox state protein; intermediate formation leads aggregation. maintains prevents aggregation protein. another early kinetics analysed presence Whereas, speeds up rate, delay rate