Stalling of Human Methyltransferase at Single-Strand Conformers from the Huntington's Locus☆
作者： Steven S. Smith , David J. Baker
关键词: DNA 、 Methylation 、 Peptide sequence 、 DNMT1 、 Genetics 、 Methyltransferase 、 Biology 、 DNA methyltransferase 、 DNA methylation 、 Locus (genetics)
摘要: We describe evidence for a sequence of events in which the Human DNA(cytosine-5)methyl-transferase first methylates spontaneous single-stranded conformers (SSCs) and then stalls at methylated site to produce complex with conformationally unusual DNA. This property enzyme is result its ability respond general loss symmetry CG recognition site. The data suggest that DNA methyltransferase, itself, may physically participate biological processes distinguish between normal Watson-Crick paired conformation (e.g. hairpin loop or misassembled replication intermediate). vitro methylation SSCs from Huntington's locus illustrates phenomenon.
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