Biodegradable quantum dot nanocomposites enable live cell labeling and imaging of cytoplasmic targets.

摘要: Semiconductor quantum dots (QDs) offer great promise as the new generation of fluorescent probes to image and study biological processes. Despite their superior optical properties, QDs for live cell monitoring tracking cytoplasmic processes remain limited due inefficient delivery methods available, altered state or function cells during process requirement surface-functionalized specific labeling subcellular structures. Here, we present a noninvasive method structures in using bioconjugated QD nanocomposites. By incorporating antibody-coated within biodegradable polymeric nanospheres, have designed bioresponsive system that undergoes endolysosomal cytosolic translocation via pH-dependent reversal nanocomposite surface charge polarity. Upon entering cytosol, polymer nanospheres undergo hydrolysis thus releasing bioconjugates. This approach facilitates multiplexed inside without fixation membrane permeabilization. As compared conventional intracellular techniques, this allows high throughput with minimal toxicity cell. More importantly, development demonstrates an important rational strategy design multifunctional nanosystem applications.