Expression pattern of the urokinase-plasminogen activator system in rat DS-sarcoma: role of oxygenation status and tumour size.

摘要: The urokinase plasminogen activator system plays a central role in malignant tumour progression. Both hypoxia and enhancement of activator, activator-receptor inhibitor type 1 have been identified as adverse prognostic factors. Upregulation or could present means by which influences Therefore, the impact on expression pattern rat DS-sarcoma vivo vitro was examined. In setting, cells were implanted subcutaneously into rats, housed under either hypoxia, atmospheric air hyperoxia. For studies, incubated for 24 h hypoxia. Urokinase analysed flow cytometry. activity measured using zymography. Plasminogen protein levels examined with ELISA. PAI-1 mRNA determined RT–PCR. express activator-receptor, vivo. is enhanced DS-sarcomas compared to normal tissues rises increasing volume. oxygenation level has no cultured solid tumours, although an increase after hypoxic challenge detectable. latter changes not detectable Hypoxia demonstrated contribute upregulation some components vitro, this effect reproducible This may indicate that serum reliable surrogate marker