Inhibition of miR-22 enhanced the efficacy of icotinib plus pemetrexed in a rat model of non-small cell lung cancer.
作者： Zhi Qiang Xue , Jia Xin Wen , Yun Xi Wang , Bin Wang , Jing Zhang
DOI: 10.22038/IJBMS.2019.39291.9320
关键词: Carcinoma 、 Immunohistochemistry 、 MTT assay 、 Apoptosis 、 Transfection 、 Medicine 、 Pemetrexed 、 Lung cancer 、 Cancer research 、 Icotinib
摘要: Objective(s): To investigate the role of miR-22 in efficacy combined icotinib (BPI-2009H) and pemetrexed (LY-231514) on tumor growth apoptosis rats with non-small cell lung cancer (NSCLC).Materials Methods: Rats were injected HCC827 cells, which transfected anti-miR-22, followed by treatment BPI-2009H and/or LY-231514. MTT assay was used to detect inhibition rate cells. qRT-PCR performed examine expression cells tissues. Moreover, immunohistochemistry Western blotting related-molecule expressions, while TUNEL staining observe tissues.Results: MiR-22 decreased after or LY-231514 a dose-dependent manner. Both increased enhanced anti-miR-22 IC50 values. Furthermore, found or/and In addition, expressions PCNA, Ki67, Bcl-2 reduced, but Bax Caspase-3 treated rats, typically those combination BPI-2009H, Conclusion: Inhibition could enhance NSCLC, providing new perspective for NSCLC therapy.
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