Automated sampling and imaging of analytes separated on thin-layer chromatography plates using desorption electrospray ionization mass spectrometry.

摘要: Modest modifications to the atmospheric sampling capillary of a commercial electrospray mass spectrometer and upgrades an in-house-developed surface positioning control software package (HandsFree TLC/MS) were used enable automated imaging analytes on within large area substrates using desorption ionization spectrometry. Sampling rhodamine dyes separated TLC plates illustrate some practical applications this system. Examples are shown for user-defined spot from bands plate (one or multiple spots), scanning complete development lane lanes), analyte in (i.e., scans with close spacing). The post data acquisition processing display aspects system also discussed.