Preloaded donor corneal lenticules in a new validated 3D printed smart storage glide for Descemet stripping automated endothelial keratoplasty

摘要: Purpose To design and validate the efficacy of three-dimensional (3D) printed smart storage glide (SSG) which is capable preserving delivering posterior lenticules for Descemet stripping automated endothelial keratoplasty (DSAEK). Methods Laboratory investigation (A) was followed by clinical validation (B). Unsuitable corneas transplantation (n=20) were used study A. These tissues cut using a standard microkeratome, punched loaded into SSG preserved 7 days in transport media. Endothelial cell density (ECD), Trypan blue Alizarin red staining morphology, thickness measurements glucose uptake, apoptosis immunostaining post preservation analysed. For B, grade (n=14) preloaded grafted patients with indications Fuchs’ dystrophy (n=8), pseudophakic bullous keratopathy (n=3), polymorphous (n=2), previous DSAEK failure (n=1). Standard included descemetorhexis under air bimanual delivery tissue infusion buffered saline solution through an anterior chamber maintainer placed at 12 o’clock position. Main outcome measures B less surgical time, best spectacle-corrected visual acuity (BSCVA), speed recovery, ECD. Results A, average ECD loss 2.30±3.21%, increased 30.80±20.85% one-third utilised during phase. showed hexagonality cells. Cell not observed expression ZO-1 noted on tissues. In 25% after 6 months. BSCVA improved to 20/25 or better within 3 months DSAEK. Mean time recorded 21 min. Conclusions This paper describes development, design, laboratory 3D helps store deliver lenticules, therefore allowing transportation quality-controlled precut