The Gap Junction Proteins β1-Connexin (Connexin-32) and β2-Connexin (Connexin-26) Can Form Heteromeric Hemichannels
作者： Kathrin A. Stauffer
DOI: 10.1016/S0021-9258(18)82720-X
关键词: Connexin 32 、 Recombinant baculovirus 、 Biology 、 Homomeric 、 Connexin 、 Connexon 、 Cell biology 、 Gap Junction Proteins 、 Ran 、 Insect cell
摘要: Two different types of gap junction proteins, β1- and β2-connexin, were expressed in insect cells, either singly or together, using infection with recombinant baculovirus. Membrane fractions enriched proteins isolated, connexons (hemichannels) solubilized detergent. These then run out on a gel filtration column which was capable partially separating the two homomeric connexons. It found that from cells co-infected both baculovirus ran together this column, whereas infected separately mixed before solubilization did not, suggesting connexin are assembled into heteromeric hemichannels.
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