Cell culture systems for stem cell research
作者： K. Meade , R.J Holley , C.L.R Merry
DOI: 10.1533/9780857092915.3.372
关键词: Cell type 、 Flexibility (engineering) 、 Nanotechnology 、 Stem cell 、 Extracellular matrix 、 Embryoid body 、 Embryonic stem cell 、 Scaffold 、 Cell culture 、 Materials science
摘要: Abstract: Embryonic stem (Es) cells have the capacity to form any cell type in adult organism. However, their propagation and differentiation is limited by current two dimensional (2d) culture techniques which offer little flexibility terms of surface structure functionalisation with bioactive molecules. Electrospinning one few fabrication methods that can create a dry non-woven mesh recreates fibrous architecture extracellular matrix (ECM). Despite versatility this well established technique, studies investigated potential electrospun meshes either maintain ES pluripotency or enhance differentiation. This chapter aims provide an overview assess research has successfully combined meshes. The will also investigate novel scaffolds may be applicable explore how potentially truly three (3D) environment.
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