AB0098 GENE EXPRESSION AND FUNCTIONAL COMPARISON BETWEEN MESENCHYMAL STEM CELLS FROM LATERAL AND MEDIAL CONDYLES OF KNEE OSTEOARTHRITIS PATIENTS

摘要: Background Osteoarthritis (OA) is the most common degenerative joint disorder, mainly afflicting weight–bearing joints, and leading cause of physical disability worldwide. Despite identified risk factors, exact pathogenesis osteoarthritis remains unclear (1). Formation mesenchymal stem cells’ (MSC) clusters their aberrant osteogenic differentiation in OA subchondral bone (SB) has been proposed as a key contributor to progression animal models (2) human hip (3). MSCs have crucial role repair but it unknown how severity affects MSC numbers or characteristics. Knee provides good model determine this knee OA, distribution damage usually asymmetrical tends be more severe medial (Med) compared lateral (Lat) compartment (4). Objectives The aim study was whether there were differences numbers, topography gene expression (GE) between Med Lat femoral condyles patients with OA. Methods Condyles obtained from that underwent total replacement (n=16; UK Ethics Committee approval, 14/YH/0087). Decalcified samples histologically evaluated for cartilage damage, sclerosis CD271+ (2). extracted SB sorted using CD271+CD45- phenotype GE analysis. Colony forming unit (CFU-F) (3), trilineage (5) population doubling (PD) assays performed. condyles. Results presented significantly higher (p Conclusion Upregulation ossification-related genes condyle suggest potential contribution sclerotic plate formation severity. Further work needed establish if biomechanical biological stimulation these can result modulation preference tissue restoration. References [1] Conaghan. Nat Rev Rheumatol (2013). [2] Zhen. (2013); [3] Campbell. Arthritis (2016); [4] Bae. Cartilage (2010). [5] Altaie. Cytotherapy (2018); [6] Hu. Mol Rep (2017); [7] Garcia-Martin. Endocrinol (2014); [8] Chiellini. Biochem Biophys Res Commun (2008) Acknowledgement CSR funded by fellowship Xunta de Galicia (Consell Cul, Educ e Ordenacion Univ). TB DM part-supported NIHR Leeds Musculoskeletal Biomed Research Centre. FOREUM. JE part-funded AO foundation start-up grant. Disclosure Interests Clara Sanjurjo Rodriguez: None declared, Thomas Baboolal: Agata Burska: Frederique Ponchel: Jehan El-Jawhari: Joseph Aderinto: Owen Wall: Hemant Pandit Grant/research support from: GSK, Consultant for: For education Bristol Myers Squibb, Dennis McGonagle Lilly, Novartis UCB, Speakers bureau: Elena Jones: declared