Functional convergence of signalling by GPI-anchored and anchorless forms of a salamander protein implicated in limb regeneration.

摘要: The GPI-anchor is an established determinant of molecular localisation and various functional roles have been attributed to it. newt GPI-anchored three-finger protein (TFP) Prod1 important regulator cell behaviour during limb regeneration, but it unclear how signals the interior cell. was expressed by transfection in cultured cells activated transcription expression matrix metalloproteinase 9 (MMP9) a pathway involving ligand-independent activation epidermal growth factor receptor (EGFR) signalling phosphorylation extracellular regulated kinase 1 2 (ERK1/2). This dependent on presence critical residues α-helical region protein. Interestingly, axolotl, salamander species that also regenerates its limbs, shown activate ERK1/2 MMP9 despite being anchorless, both axolotl co-immunoprecipitated with EGFR after transfection. substitution helical secreted, anchorless version molecule. activity molecule cannot therefore depend unique property conferred anchor. salamander-specific TFP interaction phylogenetically conserved has implications for our view regeneration as evolutionary variable.